Coding

Part:BBa_K1321097:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-07)


CBDcenA-linker fused to Phytochelatin (PC) EC20


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 372


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

Phytochelatin and CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References